A Method for Investigating Protein−Protein Interactions Related to Salmonella Typhimurium Pathogenesis
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چکیده
منابع مشابه
A method for investigating protein-protein interactions related to salmonella typhimurium pathogenesis.
We successfully modified an existing method to investigate protein-protein interactions in the pathogenic bacterium Salmonella enterica serovar Typhimurium (Salmonella Typhimurium). This method includes (i) addition of a histidine-biotin-histidine tag to the bait proteins via recombinant DNA techniques, (ii) in vivo cross-linking with formaldehyde, (iii) tandem affinity purification of bait pro...
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BACKGROUND: Among all common techniques in sitedirectedmutagenesis, λ Red recombinase system has beenwidely used to knock out chromosomal genes in bacteria. In thismethod, there is always the risk of DNA Linear digestion byhost's restriction enzymes that leads to the low frequency ofrecombination. OBJECTIVES:To overcome this, we constructeda recombinant vector to disrupt phoP gene in Salmonella...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2009
ISSN: 1535-3893,1535-3907
DOI: 10.1021/pr800865d